Abstract: A single-electrode nylon fibres enhanced polytetrafluoroethylene (PTFE) electret flexible film with hollow cylinder structure for mechanical energy harvesting is reported. The power was generated by the enhanced electret PTFE with an electrostatic effect between the conductor carbon black silicone rubber mixture electrode film and a surface of the deformation PTFE film. It was found that the nylon fibres enhanced PTFE electret film can get about 1.45 times Voc than the pure PTFE with hollow cylinder structure during the mechanical energy harvesting. We also found that with the distance of travel is linear related with the peak value of short-circuit current and open-circuit voltage. A random test using finger tap to light 8 light-emitting diode was demonstrated the mechanical energy conversion performance of the prototype with the generated maximum peak short-circuit current 0.35 μA and an open-circuit voltage 72 V, respectively. At the end, an ultrasonic test was conducted to demonstrate the quick response and flexibility of the prototype at the extra high frequency.